There are many reports on the distribution of normal flora in the vagina and the
causative agents of vaginal inflammatory disease. The most commonly encountered
organisms are known to be doderlein bacillus which plays a major role in the
cleanliness of the vagina. The other organisms frequently found in healthy as well as
inflammatory vagina are staphylococci, streptococci, hemophilus species, diphtheroid
bacilli, coliforms, fungi, and others. However the incidences of lactobacilli on the cultures
show some differences between several authors. Also it is considered to be significant
relationship of lactobacilli to the vaginas acidity as welt as to vaginitis. Therefore this
study is aimed to observe the distribution of bacterial flora in nominal group and
vaginitis group, and the relation of doderlein bacilli to vaginal cleanliness and pH, were
analysed.
The results of the study were as follows;
1) The flora on direct vaginal smear in normal and vaginitis was graded according to
the presence of doderlein bacilli. Cases of normal group reveals mostly grade I (38%),
while vaginitis group reveals mostly grade Ⅱ (42%).
2) 88% of the cases isolate on the culture of the vaginal flora in normal group and
90% of the cases isolate on the culture of the vaginal flora in vaginitis group.
3) The incidence of staphylococci, coliform group, hemophilus species and monilia in
vaginitis group are higher than that of normal group, while the incidence of lactobacilli
and diphtheroid bacilli in vaginitis group are lower than that of normal group.
4) 56% in normal group and 43% in vaginitis group grew on general culture media
with 5-l0% Co2. But 79% in normal group and 58% in vaginitis group of
lactobacilli grew on selective media with 5-l0% Co2, Also cultivation of
vaginal lactobacilli on selective media gives satisfactory yield.
5) The distribution of vaginal pH values in vaginitis group reveals higher pH values
than that of normal group. In the normal, vaginal pH values were distributed 4-5
predominantly, and vaginal pH values of vaginitis were distributed 5-6 predominantly.
6) Lower the pH value of vagina, lower the grading of direct vaginal smear, and also
increase the positivity of lactobacilli on selective media.
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